Mesenchymal Stem Cell Differentiation Driven by Osteoinductive Bioactive Nanoscale Topographies

Human mesenchymal stem cells (hMSCs) respond to the characteristics of their surrounding microenvironment, i.e., extracellular matrix (ECM). The possibility mimicking ECM offers opportunity elicit specific cell behaviors. control surface properties a biomaterial at scale level components has potential effectively modulate response. Ordered nanoscale silicon pillar arrays were fabricated using reverse micelles block copolymers on full wafers, with standard deviations lower than 15%. Bioactive synthetic peptides covalently grafted nanoarrays evaluate possible synergies between chemistry and topography osteogenic differentiation hMSCs. Functionalization RGD (Arg-Gly-Asp) BMP-2 (bone morphogenetic protein-2) mimetic lead an enhancement differentiation. Bare nanopillar reduced pitch found promote faster hMSC These findings highlight relevance investigating possibilities engineering in vitro systems which can be fine-tuned according envisaged